Automating a 96-well microtiter plate assay for advanced glycation end-products inhibitors or inducers identification: application to the screening of a small natural compounds library.

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TitreAutomating a 96-well microtiter plate assay for advanced glycation end-products inhibitors or inducers identification: application to the screening of a small natural compounds library.
Type de publicationArticle de revue
AuteurDerbré, Séverine , Gatto, Julia , Pelleray, Aude , Coulon, Laurie , Seraphin, Denis , Richomme, Pascal
EditeurSpringer Verlag
TypeArticle scientifique dans une revue à comité de lecture
Année2010
LangueAnglais
Numéro4
Pagination1747-1758
Volume398
Titre de la revueAnalytical and Bioanalytical Chemistry
ISSN1618-2642
Mots-clésAdvanced glycation end-products, aging automation, biological sceening, Diabetes, natural products
Résumé en anglais

Advanced glycation end-products (AGEs) are involved in the pathogenesis of numerous affections such as diabetes and neurological diseases. AGEs are also implied in various changes in tissues and organs. Therefore, compounds able to break them or inhibit their formation may be considered as potential drugs, dietary supplements, or bioactive additives. In this study, we have developed a rapid and reliable (Z′ factor calculation) anti-AGEs activity screening based on the overall fluorescence of AGEs. This method was successfully evaluated on known AGEs inhibitors and on a small library of natural compounds, yielding coherent results when compared with literature data.

URL de la noticehttp://okina.univ-angers.fr/publications/ua10637
DOI10.1007/s00216-010-4065-1
Titre abrégéAnal. Bioanal. Chem.