Understanding the adsorption of salmon calcitonin, antimicrobial peptide AP114 and polymyxin B onto lipid nanocapsules

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TitreUnderstanding the adsorption of salmon calcitonin, antimicrobial peptide AP114 and polymyxin B onto lipid nanocapsules
Type de publicationArticle de revue
AuteurUmerska, Anita-Monika , Matougui, Nada , Groo, Anne-Claire , Saulnier, Patrick
PaysPays-Bas
EditeurElsevier
TypeArticle scientifique dans une revue à comité de lecture
Année2016
LangueAnglais
Date15 juin 2016
Numéro1-2
Pagination191-200
Volume506
Titre de la revueInternational journal of pharmaceutics
ISSN1873-3476
Mots-clésAdsorption, Antimicrobial peptide, AP114, Calcitonin, Lipid nanocapsules, nanoparticles, NZ2114, Peptide, Plectasin, Polymyxin B
Résumé en anglais

The adsorption of therapeutic molecules, e.g., peptides, onto nanocarriers is influenced by the properties of the carrier, adsorbed molecule and continuous phase. Hence, through changes in the composition of the nanocarrier and the medium, it should be possible to tune the system to make it capable of efficiently adsorbing peptides. The adsorption of calcitonin, antimicrobial peptide AP114 and polymyxin B onto lipid nanocapsules was investigated. The adsorption data were fitted to a Langmuir isotherm. Dynamic light scattering and laser Doppler velocimetry were used to investigate the changes in the hydrodynamic diameter and zeta potential, respectively, of the nanocarrier. The peptide adsorption was primarily governed by electrostatic forces; however, even without the presence of an ionisable surfactant, a significant amount of each tested molecule was adsorbed due to the enormous surface area of the nanocarriers and to peptide-nanocarrier interactions. The addition of an ionisable lipophilic surfactant, lecithin, improved the adsorption yield, which reached values of up to 100%. The adsorption yield and the properties of the nanocarrier, particularly the zeta potential, depended on the carrier and peptide concentrations and their mixing ratio. The adsorption of all tested molecules obeyed the Langmuir model over a limited concentration range.

URL de la noticehttp://okina.univ-angers.fr/publications/ua14647
DOI10.1016/j.ijpharm.2016.04.028
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http://www.sciencedirect.com/science/article/pii/S0378517316303143

Autre titreInt J Pharm
Identifiant (ID) PubMed27113868