Lipid nanocapsules maintain full integrity after crossing a human intestinal epithelium model

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TitreLipid nanocapsules maintain full integrity after crossing a human intestinal epithelium model
Type de publicationArticle de revue
AuteurRoger, Emilie , Gimel, Jean-Christophe , Bensley, Conor , Klymchenko, Andrey S , Benoît, Jean-Pierre
EditeurElsevier
TypeArticle scientifique dans une revue à comité de lecture
Année2017
LangueAnglais
Pagination11-18
Volume253
Titre de la revueJournal of Controlled Release
ISSN0168-3659
Mots-clésCaco-2 Intestinal transport, FRET, Integrity, Nanocapsules
Résumé en anglais

Lipid nanocapsules (LNCs) have demonstrated great potential for the oral delivery of drugs having very limited oral bioavailability (BCS class II, III and IV molecules). It has been shown previously that orally-administered LNCs can permeate through mucus, increase drug absorption by the epithelial tissue, and finally, increase drug bioavailability. However, even if transport mechanisms through mucus and the intestinal barrier have already been clarified, the preservation of particle integrity is still not known. The aim of the present work is to study in vitro the fate of LNCs after their transportation across an intestinal epithelium model (Caco-2 cell model). For this, two complementary techniques were employed: Förster Resonance Energy Transfer (FRET) and Nanoparticle Tracking Analysis (NTA). Results showed, after 2 h, the presence of nanoparticles in the basolateral side of the cell layer and a measurable FRET signal. This provides very good evidence for the transcellular intact crossing of the nanocarriers.

URL de la noticehttp://okina.univ-angers.fr/publications/ua15674
DOI10.1016/j.jconrel.2017.03.005
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http://www.sciencedirect.com/science/article/pii/S0168365917301098