Synergistic Effect of Combinations Containing EDTA and the Antimicrobial Peptide AA230, an Arenicin-3 Derivative, on Gram-Negative Bacteria

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TitreSynergistic Effect of Combinations Containing EDTA and the Antimicrobial Peptide AA230, an Arenicin-3 Derivative, on Gram-Negative Bacteria
Type de publicationArticle de revue
AuteurUmerska, Anita-Monika , Strandh, Magnus, Cassisa, Viviane , Matougui, Nada , Eveillard, Matthieu , Saulnier, Patrick
EditeurMDPI
TypeArticle scientifique dans une revue à comité de lecture
Année2018
LangueAnglais
Date23 Oct. 2018
Numéro4
Pagination122
Volume8
Titre de la revueBiomolecules
ISSN2218-273X
Mots-clésAcinetobacter baumannii, Antibiotic resistance, antimicrobial peptides, arenicin, EDTA, Escherichia coli, ESKAPE pathogens, Gram-negative bacteria, Pseudomonas aeruginosa, synergy
Résumé en anglais

The worldwide occurrence of resistance to standard antibiotics and lack of new antibacterial drugs demand new strategies to treat complicated infections. Hence, the aim of this study was to examine the antibacterial activities of an antimicrobial peptide, arenicin-3 derivative AA230, and ethylenediaminetetraacetic acid (EDTA) as well as the two compounds in combination against Gram-negative bacteria. AA230 showed strong antibacterial activity against all of the studied standard strains and clinical isolates, with minimum inhibitory concentrations ranging between 1 µg/mL and 8 µg/mL. AA230 exhibited a bactericidal mode of action. EDTA inhibited the growth of at 500⁻1000 µg/mL. Strains of were found to be more susceptible to EDTA than or . The antibacterial effects of both AA230 and EDTA were independent of the antibiotic resistance patterns. Indifference to synergistic activity was observed for AA230 and EDTA combinations using checkerboard titration. In time-kill studies, a substantial synergistic interaction between AA230 and EDTA was detected against all of the tested strains. The addition of EDTA enabled a 2⁻4-fold decrease in the AA230 dose. In conclusion, AA230 could have potential applications in the treatment of infections caused by Gram-negative organisms, and its effect can be potentiated by EDTA.

URL de la noticehttp://okina.univ-angers.fr/publications/ua18089
DOI10.3390/biom8040122
Lien vers le document

https://www.mdpi.com/2218-273X/8/4/122

Autre titreBiomolecules
Identifiant (ID) PubMed30360557