Preparation and evaluation of trityl-loaded lipid nanocapsules as oxygen sensors for electron paramagnetic resonance oximetry

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TitrePreparation and evaluation of trityl-loaded lipid nanocapsules as oxygen sensors for electron paramagnetic resonance oximetry
Type de publicationArticle de revue
AuteurNel, Janske
1, 2
, Desmet, Céline M , Driesschaert, Benoit , Saulnier, Patrick , Lemaire, Laurent , Gallez, Bernard
EditeurElsevier
TypeArticle scientifique dans une revue à comité de lecture
Année2019
LangueAnglais
Pagination87-92
Volume554
Titre de la revueInternational Journal of Pharmaceutics
ISSN1873-3476
Mots-cléselectron paramagnetic resonance, Hypoxia, Lipid nanocapsules, Nitroxide, Oximetry, Trityl
Résumé en anglais

Oxygen is essential in physiology and pathophysiology. Electron paramagnetic resonance (EPR) oximetry, using oxygen sensitive paramagnetic materials, could be attractive for measuring oxygen in tissues. The aim of the present study was to assess the properties of lipid nanocapsules (LNCs) loaded with the nitroxide tempo-benzoate (TB) or tetrathiatriarylmethyl (TAM) radicals. LNCs loaded with the EPR probes were successfully prepared by the phase inversion process leading to nanocapsules of about 60 nm. LNCs protected the TB radical against reduction in vitro. The calibration of the EPR line width (LW) as a function of the pO showed a two-fold increase in sensitivity with TAM-LNC compared to hydrophilic trityl radical. The TAM-LNCs were evaluated in vivo. Contrarily to unencapsulated TAM, for which a rapid decrease in EPR signal was observed, the half-life of TAM-LNCs administered in muscles or in tumours exceeded an hour. Carbogen-challenges in mice demonstrated that the TAM-LNCs responded well to changes in oxygen environment. However, the apparent pO values acquired were higher than the expected physiological values. These results warrant further investigation in the formulation of stable nano-objects encapsulating EPR oxygen sensitive probes.

URL de la noticehttp://okina.univ-angers.fr/publications/ua18090
DOI10.1016/j.ijpharm.2018.11.007
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https://www.sciencedirect.com/science/article/pii/S0378517318308251?via%...

Titre abrégéInt J Pharm
Identifiant (ID) PubMed30399436