SiRNA LNCs - A novel platform of lipid nanocapsules for systemic siRNA administration

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TitreSiRNA LNCs - A novel platform of lipid nanocapsules for systemic siRNA administration
Type de publicationArticle de revue
AuteurDavid, Stephanie, Resnier, Pauline , Guillot, A., Pitard, Bruno, Benoît, Jean-Pierre , Passirani-Malleret, Catherine
TypeArticle scientifique dans une revue à comité de lecture
DateJuin 2012
Titre de la revueEuropean Journal of Pharmaceutics and Biopharmaceutics
Mots-clésCationic lipids, Formulation, Lipoplexes, Nanocarrier, siRNA quantification, Systemic administration
Résumé en anglais

Several siRNA (small interfering RNA) therapeutics are undergoing clinical trials for cancer, respiratory diseases or macular degeneration, but most are administrated locally. In order to overcome the different barriers to attain an efficient siRNA action after systemic administration, nanocarriers able to carry and protect siRNA are awaited. With this aim, we developed a new platform of siRNA lipid nanocapsules (LNCs) using different cationic lipids, combining the properties of LNCs (siRNA protection and targeting) and lipoplexes (efficient siRNA delivery into the cell). The formulation was revealed to contain different compartments. A siRNA quantification method based on UV spectroscopy was developed to locate and quantify siRNA in each compartment. All in all, these novel siRNA LNCs presented sizes of about 55 nm with a neutral surface charge and siRNA encapsulation efficiencies up to 65% representing appropriate characteristics for systemic administration.

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