Molecular Mechanisms of Resistance to 5-Fluorocytosine in Laboratory Mutants of Candida glabrata

TitreMolecular Mechanisms of Resistance to 5-Fluorocytosine in Laboratory Mutants of Candida glabrata
Type de publicationArticle de revue
AuteurVandeputte, Patrick , Pineau, Laurent, Larcher, Gérald , Noel, Thierry, Brèthes, Daniel, Chabasse, Dominique , Bouchara, Jean-Philippe
EditeurSpringer Verlag
TypeArticle scientifique dans une revue à comité de lecture
Pagination11 - 21
Titre de la revueMycopathologia
Mots-clés5-Fluorocytosine, Candida glabrata, Eukaryotic Microbiology, FUR1, FCY1 and FCY2 genes, Medical Microbiology, Microbial Ecology, Microbiology, Plant Sciences, Resistance mechanisms
Résumé en anglais

Resistance to 5-fluorocytosine (5-FC) has been poorly investigated in the yeast Candida glabrata. This study was conducted on laboratory mutants obtained by exposure of a wild-type isolate to 5-FC. Based on their susceptibility to 5-fluorouracil (5-FU), two of these mutants were selected for further analysis of the molecular mechanisms of 5-FC resistance. One mutant, resistant to both compounds, exhibited a missense mutation in the gene coding the cytosine deaminase and a decrease in the expression level of the gene coding the uridine monophosphate pyrophosphorylase. The other mutant that showed a reduced susceptibility to 5-FC and 5-FU exhibited an overexpression of the genes coding the thymidylate synthase and a cytosine permease, associated with a missense mutation in the last gene. Thus, beside mutations in the FUR1 gene which represent the most common cause of resistance to 5-FC, other mechanisms may also occur in C. glabrata.

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