Molecular Mechanisms of Resistance to 5-Fluorocytosine in Laboratory Mutants of Candida glabrata

TitreMolecular Mechanisms of Resistance to 5-Fluorocytosine in Laboratory Mutants of Candida glabrata
Type de publicationArticle de revue
AuteurVandeputte, Patrick , Pineau, Laurent, Larcher, Gérald , Noel, Thierry, Brèthes, Daniel, Chabasse, Dominique , Bouchara, Jean-Philippe
EditeurSpringer Verlag
TypeArticle scientifique dans une revue à comité de lecture
Année2011
LangueAnglais
Date2011/01/01
Numéro1
Pagination11 - 21
Volume171
Titre de la revueMycopathologia
ISSN1573-0832
Mots-clés5-Fluorocytosine, Candida glabrata, Eukaryotic Microbiology, FUR1, FCY1 and FCY2 genes, Medical Microbiology, Microbial Ecology, Microbiology, Plant Sciences, Resistance mechanisms
Résumé en anglais

Resistance to 5-fluorocytosine (5-FC) has been poorly investigated in the yeast Candida glabrata. This study was conducted on laboratory mutants obtained by exposure of a wild-type isolate to 5-FC. Based on their susceptibility to 5-fluorouracil (5-FU), two of these mutants were selected for further analysis of the molecular mechanisms of 5-FC resistance. One mutant, resistant to both compounds, exhibited a missense mutation in the gene coding the cytosine deaminase and a decrease in the expression level of the gene coding the uridine monophosphate pyrophosphorylase. The other mutant that showed a reduced susceptibility to 5-FC and 5-FU exhibited an overexpression of the genes coding the thymidylate synthase and a cytosine permease, associated with a missense mutation in the last gene. Thus, beside mutations in the FUR1 gene which represent the most common cause of resistance to 5-FC, other mechanisms may also occur in C. glabrata.

URL de la noticehttp://okina.univ-angers.fr/publications/ua4970
DOI10.1007/s11046-010-9342-1
Lien vers le document

http://dx.doi.org/10.1007/s11046-010-9342-1