Pro-inflammatory effect of cystic fibrosis sputum microparticles in the murine lung

TitrePro-inflammatory effect of cystic fibrosis sputum microparticles in the murine lung
Type de publicationArticle de revue
AuteurPorro, Chiara, Di Gioia, Sante, Trotta, Teresa, Lepore, Silvia, Panaro, Maria Antonietta, Battaglino, Anna, Ratclif, Luigi, Castellani, Stefano, Bufo, Pantaleo, Martinez, Maria Carmen , Conese, Massimo
EditeurElsevier
TypeArticle scientifique dans une revue à comité de lecture
Année2013
LangueAnglais
Date2013 Dec
Pagination721-728
Volume12
Titre de la revueJournal of Cystic Fibrosis
ISSN1873-5010
Mots-clésAdult, Animals, Antigens, CD, Bronchoalveolar Lavage Fluid, Cell Adhesion Molecules, Cell-Derived Microparticles, Cystic fibrosis, Humans, Lipopolysaccharides, Mice, Sputum, Young Adult
Résumé en anglais

BACKGROUND: The role of microparticles (MPs) in the inflammatory process of cystic fibrosis (CF) airways is not known. Here, we have studied the pro-inflammatory potential of CF MPs in a model of acute lung injury.

METHODS: Swiss mice were subjected to intratracheal administration of MPs obtained from CF and primary ciliary diskinesia (PCD) patients. Histopathology, total and differential cell counts in bronchoalveolar lavage fluid were used to evaluate the inflammatory reaction in the lung. Lipopolysaccharide (LPS)-like activity of MPs was studied by Limulus amebocyte lysate assay.

RESULTS: MPs obtained from acute CF patients determined peribronchial and perivascular inflammatory infiltrates similar to those elicited by LPS. This inflammation was granulocyte-dominated and higher than that determined by MPs obtained from stable CF, whereas PCD MPs caused a macrophage-dominated inflammation. While LPS-activity was not found in circulating blood MPs prepared from CF patients, it was present in MPs obtained from CF sputum and sputum CD66b(+) neutrophils. Finally, LPS-like activity was only detected in circulating MPs after incubation with LPS as well as in MPs obtained from LPS-stimulated neutrophils obtained from healthy donors.

CONCLUSIONS: These data suggest that the pro-inflammatory potential of neutrophil-derived MPs in the CF airways may be subsequent to the binding of shedded LPS.

URL de la noticehttp://okina.univ-angers.fr/publications/ua10567
DOI10.1016/j.jcf.2013.03.002
Titre abrégéJ. Cyst. Fibros.
Identifiant (ID) PubMed23567201