Influence of size, surface coating and fine chemical composition on the in vitro reactivity and in vivo biodistribution of lipid nanocapsules versus lipid nanoemulsions in cancer models

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TitreInfluence of size, surface coating and fine chemical composition on the in vitro reactivity and in vivo biodistribution of lipid nanocapsules versus lipid nanoemulsions in cancer models
Type de publicationArticle de revue
AuteurHirsjärvi, Samuli , Dufort, Sandrine, Gravier, Julien, Texier, Isabelle, Yan, Qiao, Bibette, Jérome, Sancey, Lucie, Josserand, Véronique, Passirani-Malleret, Catherine , Benoît, Jean-Pierre , Coll, Jean-Luc
EditeurElsevier
TypeArticle scientifique dans une revue à comité de lecture
Année2013
LangueAnglais
DateAvril 2013
Numéro3
Pagination375-87
Volume9
Titre de la revueNanomedicine
ISSN1549-9642
Mots-clésAnimals, Coated Materials, Biocompatible, complement activation, Drug Carriers, Emulsions, Endocytosis, Female, HEK293 Cells, Humans, Lipids, Mice, Mice, Nude, Nanocapsules, Neoplasms, Particle Size, Subcutaneous Tissue, Surface Properties, Tissue Distribution, Xenograft Model Antitumor Assays
Résumé en anglais

UNLABELLED: Lipid nanocapsules (LNCs) and lipid nanoemulsions (LNEs) are biomimetic synthetic nanocarriers. Their in vitro and in vivo performance was evaluated as a function of their size (25, 50 and 100 nm) and the surface PEG chain length. Analysis methods included complement activation test, particle uptake in macrophage and HEK293(β3) cells and biodistribution studies with tumor-grafted mice by fluorescence imaging. A particular attention was paid to keep the concentration of each nanocarrier and to the amount of fluorescent dye in comparable conditions between the in vitro and in vivo studies. Under these conditions, no significant differences were found among the three tested particle sizes and the two nanocarrier types. Longer PEG chains on the LNE surface provided better stealth properties, whereas PEG modification on the LNC formulations inhibited the production of stable nanocarriers. Passive accumulation of LNCs and LNEs in different tumor types depended on the degree of tumor vascularization.

FROM THE CLINICAL EDITOR: This study of lipid nanocapsules and lipid nanoemulsions compares their vitro and in vivo performance as a function of size and surface PEG chain length, demonstrating no significant difference among the tested particle sizes. Longer PEG chains on the LNE surface provided better stealth properties, whereas PEG modification on the LNC formulations inhibited the production of stable nanocarriers.

URL de la noticehttp://okina.univ-angers.fr/publications/ua14337
DOI10.1016/j.nano.2012.08.005
Autre titreNanomedicine: nanotechnology, biology and medicine
Identifiant (ID) PubMed22960195