Performance of the extended use of the FilmArray BCID panel kit for bronchoalveolar lavage analysis

TitrePerformance of the extended use of the FilmArray BCID panel kit for bronchoalveolar lavage analysis
Type de publicationArticle de revue
AuteurSansot, Maxime, Fradin, Eugénie, Chenouard, Rachel, Kempf, Marie , Kouatchet, Achille, Lasocki, Sigismond , Lemarié, Carole, Eveillard, Matthieu , Pailhories, Hélène
EditeurSpringer (part of Springer Nature)
TypeArticle scientifique dans une revue à comité de lecture
Année2019
LangueAnglais
DateJuin 2019
Numéro3
Pagination2685-2692
Volume46
Titre de la revueMolecular Biology Reports
ISSN1573-4978
Mots-clésDiagnosis, Intensive Care Units, Multiplex PCR, Nosocomial infection, Ventilator associated pneumonia
Résumé en anglais

Ventilator-associated pneumonia (VAP) are responsible for an increase in morbidity, mortality, and prolonged hospital stay. A multiplex PCR kit such as the FilmArray BCID panel could allow early adaptation of antimicrobial therapy, which is crucial for clinical outcomes. The purpose of this study was to test the performances of FilmArray BCID panel for the detection of bacteria producing VAP. We tested the FilmArray BCID panel on 50 bronchoalveolar lavages (BALs), from patients hospitalized in two intensive care units at the Angers university hospital, compared to the conventional culture-based method. The sensitivity and the specificity of the FilmArray BCID panel were 67.2% and 98.9% respectively. They were 88.6% and 98.3% respectively when considering BALs with a positive culture > 10 CFU/mL, and 94.7% and 99.6% respectively if considering BALs with a positive direct examination. This study underlines the good performance of the FilmArray BCID panel for BAL fluid analysis. In case of positive direct examination, this test allows reliable results that can be obtained at an early stage, facilitating the early adaptation of antimicrobial therapy.

URL de la noticehttp://okina.univ-angers.fr/publications/ua19476
DOI10.1007/s11033-019-04710-0
Lien vers le document

https://link.springer.com/article/10.1007%2Fs11033-019-04710-0

Titre abrégéMol. Biol. Rep.
Identifiant (ID) PubMed30798486