Normal phase HPLC profiling of the acetylcholinesterase activity in apolar plant extracts

TitreNormal phase HPLC profiling of the acetylcholinesterase activity in apolar plant extracts
Type de publicationCommunication
TypeCommunication par affiche dans un congrès
Date du colloque28-31/07/2012
Titre du colloqueInternational Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF
Paginationp. 1262
AuteurLandreau, Anne , Bertrand, Samuel, Simoes-Pires, Claudia, Marcourt, Laurence, Bach, T-D, Litaudon, Marc , Guilet, David , Richomme, Pascal , Carrupt, Pierre-Alain, Wolfender, Jean-Luc
EditeurPlanta Medica
Résumé en anglais

Among nineteen evaluated Clusiaceous species, one stem bark CH2Cl2 crude extract was selected based on a significant inhibition of acetylcholinesterase (AChE) using the micro-dilution Ellman's method [1]. A normal phase HPLC profiling with micro-fractionation of this extract provided discrete fractions every 20 seconds. In order to obtain a comprehensive profiling of AChE activity all microfractions were tested [2] in dilution assay (Ellman) as well as by bioautography (the Fast Blue B salt method). Furthermore the potency of inhibition was evaluated both by keeping the genuine concentration within the extract and after normalisation to a standard concentration level. From the active fractions five pure compounds were isolated and identified. The different methods of sample preparation and biological evaluation associated with normal-phase micro-fractionation of plant extracts are critically discussed.

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