Titre | Nitric Oxide and Calcium Participate in the Fine Regulation of Mitochondrial Biogenesis in Follicular Thyroid Carcinoma Cells |
Type de publication | Article de revue |
Auteur | Le Pennec, Soazig, Mirebeau-Prunier, Delphine , Bouzamondo, Nathalie, Jacques, Caroline , Guillotin, Delphine, Lauret, Emilie, Houlgatte, Rémi, Malthièry, Yves , Savagner, Frédérique |
Editeur | American Society for Biochemistry and Molecular Biology |
Type | Article scientifique dans une revue à comité de lecture |
Année | 2011 |
Langue | Anglais |
Date | 2011/05 |
Numéro | 20 |
Pagination | 18229 - 18239 |
Volume | 286 |
Titre de la revue | Journal of Biological Chemistry |
ISSN | 0021-9258, 1083-351X |
Mots-clés | Calcium, Gene, mitochondrial, Nitric, thyroid |
Résumé en anglais | Members of the peroxisome proliferator-activated receptor γ coactivator-1 family (i.e. PGC-1α, PGC-1β, and the PGC-1-related coactivator (PRC)) are key regulators of mitochondrial biogenesis and function. These regulators serve as mediators between environmental or endogenous signals and the transcriptional machinery governing mitochondrial biogenesis. The FTC-133 and RO82 W-1 follicular thyroid carcinoma cell lines, which present significantly different numbers of mitochondria, metabolic mechanisms, and expression levels of PRC and PGC-1α, may employ retrograde signaling in response to respiratory dysfunction. Nitric oxide (NO) and calcium have been hypothesized to participate in this activity. We investigated the effects of the S-nitroso-N-acetyl-dl-penicillamine-NO donor, on the expression of genes involved in mitochondrial biogenesis and cellular metabolic functions in FTC-133 and RO82 W-1 cells by measuring lactate dehydrogenase and cytochrome c oxidase (COX) activities. We studied the action of ionomycin and 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid-acetoxymethyl ester (BAPTA/AM) (i.e. a calcium ionophore and a cytosolic calcium chelator) on whole genome expression and mitochondrial biogenesis in RO82 W-1 cells. COX activity and the dynamics of endoplasmic reticulum and mitochondrial networks were analyzed in regard to calcium-modulating treatments. In the FTC-133 and RO82 W-1 cells, the mitochondrial biogenesis induced by NO was mainly related to PRC expression as a retrograde mitochondrial signaling. Ionomycin diminished COX activity and negatively regulated PRC-mediated mitochondrial biogenesis in RO82 W-1 cells, whereas BAPTA/AM produced the opposite effects with a reorganization of the mitochondrial network. This is the first demonstration that NO and calcium regulate mitochondrial biogenesis through the PRC pathway in thyroid cell lines. |
URL de la notice | http://okina.univ-angers.fr/publications/ua331 |
DOI | 10.1074/jbc.M110.217521 |
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