Disuse induced by botulinum toxin affects the bone marrow expression profile of bone genes leading to a rapid bone loss.

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TitreDisuse induced by botulinum toxin affects the bone marrow expression profile of bone genes leading to a rapid bone loss.
Type de publicationArticle de revue
AuteurLibouban, H., Le Drévo, M.-A. , Chappard, Daniel
EditeurInternational Society of Musculoskeletal and Neuronal Interactions
TypeArticle scientifique dans une revue à comité de lecture
Date2013 Mar
Titre de la revueJ Musculoskelet Neuronal Interact
Mots-clésAnimals, Bone Marrow, Bone resorption, Botulinum Toxins, Female, Mice, Muscular Disorders, Atrophic, Quadriceps Muscle, Transcriptome
Résumé en anglais

OBJECTIVES: Molecular events occurring in the bone marrow microenvironment of an immobilized mouse limb after Botulinum toxin (BTX) injection haven't been characterized. BTX injection induces a localized disuse in which the tissue events have well been characterized.

METHODS: BTX injection was performed in the right quadriceps; saline injection in the left side was used as control. Mice were sacrificed at 0, 7, 14, 21 and 28 days; tibias were used for microCT analysis; bone marrow from femurs for RT-PCR analysis.

RESULTS: MicroCT revealed bone loss and microarchitectural damages on the immobilized side as from 7d; cortical area tended to be lower on the immobilized limb at 28d. Gene expression of formation factors was altered as from 7 days post-BTX: alkaline phosphatase, Tgfβ1, Lrp5, Sfrp2. Only Sfrp2 and Lrp5 were maintained altered until 28d. Expression of Dkk1 increased from 21d and represented a late inhibitor of formation. Gene expression of resorption markers increased as from 7d (Rankl, Tracp, Il1α, Il1β and Il6) and was maintained until 28d for Tracp and Il6.

CONCLUSION: A localized disuse induces rapid modifications in the bone marrow gene expression leading to bone loss due to an early decrease of formation associated with an increase in resorption.

URL de la noticehttp://okina.univ-angers.fr/publications/ua3443
Autre titreJ Musculoskelet Neuronal Interact
Identifiant (ID) PubMed23445912