Hydroxamate siderophores of Scedosporium apiospermum

TitreHydroxamate siderophores of Scedosporium apiospermum
Type de publicationArticle de revue
AuteurBertrand, Samuel, Larcher, Gérald , Landreau, Anne, Richomme, Pascal , Duval, Olivier, Bouchara, Jean-Philippe
EditeurSpringer Verlag
TypeArticle scientifique dans une revue à comité de lecture
Pagination1019 - 1029
Titre de la revueBioMetals
Mots-clésBiochemistry, general, Dimerumic acid, Hydroxamate-type siderophores, N, Physical Chemistry, Scedosporium apiospermum, α-methyl coprogen B
Résumé en anglais

Scedosporium apiospermum is an emerging pathogen colonizing the airways of patients with cystic fibrosis and causing severe infections in immunocompromised hosts. In order to improve our knowledge on the pathogenic mechanisms of this fungus, we investigated the production of siderophores. Cultivation on CAS medium and specific assays for different classes of siderophores suggested the secretion of hydroxamates. A maximal production was obtained by cultivation of the fungus at alkaline pH in an iron-restricted liquid culture medium. Siderophores were then extracted from the culture filtrate by liquid/liquid extraction, and separated by reverse phase high performance liquid chromatography. Two siderophores, dimerumic acid and N α-methyl coprogen B, were identified by electrospray ionization-mass spectrometry and MS–MS fragmentation. Finally, comparison of various strains suggested a higher production of N α-methyl coprogen B by clinical isolates of respiratory origin. Studies are initiated in order to determine the potential usefulness of these siderophores as diagnostic markers of scedosporiosis.

URL de la noticehttp://okina.univ-angers.fr/publications/ua4876
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